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To visually observe the live and dead cells induced by different nanocomposites, we stained the cells with Calcein AM (acetoxymethyl ester of calcein, green fluorescence indicates live cells) and propidium iodide (PI, red fluorescence indicates dead cells) [36]

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While this is a single case and cannot determine class effects, it is a data point worth noting

If the cartilage is still intact, a surgeon can perform procedures to release tight structures, transfer tendons, and tighten loose ligaments to rebalance the joint alignment and improve function